|
Differential Media and Their Uses |
| Medium |
Purpose |
Notes |
| Lysine medium (LYS) |
Detection of wild yeast |
Lactic acid solution to be added after boiling, when temperature is below 50°C. |
| Schwartz Differential Medium (SDM) |
Detection of wild yeast |
Prestorage of plates is needed before use; not to be confused with SDA-Schwartz Differential Agar |
| Lin's Wild Yeast Medium (LWYM) |
Detection of wild yeast |
Should be used within 5 days of preparation. To detect wild Saccharomyces spp., add crystal violet. Some cultured yeast may show slight growth in this medium, so only distinct colonies should be counted. |
| Lin's Cupric Sulfate Medium (LCSM) |
Detection of wild yeast |
Use within 3 days of preparation. Also detects wild Saccharomyces spp. |
| TCC overlay |
Detection of respiratory deficient mutants (RDMs) |
. |
| HLP medium |
Detection of Lactobacillus and Pediococcus bacteria specifically |
Also known as Hsu's Lactobassillus and Pediococcus medium.. |
| Wort agar |
To grow both yeast and bacteria |
General agar with wort added for nutrients |
| Hsu's Rapid Growth Medium (HRM) |
For the rapid growth of yeast and bacteria. To be used when looking for microorganisms in cooled, nonpitched wort |
|
| Wallerstein Labs Differential Agar (WLD agar) |
For detection of RD mutants |
Contains brom cresol green. Smaller, darker green colonies are respiratory deficient |
| Universal Beer Agar (UBA) |
For detection of Brettanomyces
wild yeast |
Contains Actidione |
